In 14 rats used as controls.

Harry G. Preys, Bobby Echard, Ryan Reza Zonosi

volatile aromatic oils and medium fatty acids derived from edible plants possess interesting antimicrobial properties - properties are generally not recognized by state or, in this regard, to the medical profession. Antimicrobial activity of oregano oil is due largely to a high content of phenolic components, such as karvakrol and thymol. Lauric acid, found primarily in coconut and palm oil, can form monolaurin in the body, monoglycerides with high antimicrobial potential. We recently compared the efficacy of antimicrobial oregano oil and other essential oils and monolaurin in vitro by studying fungi (C Albicans) and a variety of bacteria by the method mikrorozveden. Oregano oil, the most powerful of the essential oils tested and proven monolaurin cidal in a culture different bacteria. However, only oregano, not monolaurin is cidal to C. Albicans. In vivo experiments in mice have shown oregano oil and monolaurin, individually and in combination to be as effective as some antibiotics. Oregano oil and / or monolaurin may prove useful antimicrobial drugs to prevent and treat a variety of infectious organisms in different circumstances. The total worldwide health problems caused by heavy, difficult to treat infectious diseases has become over time. While the standard approach is to use drugs, medications was not a complete solution. Too many deadly microbes created a resistance to many antibiotics still strong, despite further development over time is more complicated antimicrobial agents. More disturbing is that many antimicrobial drugs were also associated with severe adverse reactions. Thus, safe and effective antimicrobial drugs for treatment of a wide range of both therapeutic and prophylactic broad spectrum of infections is needed. This is especially true for many treatment-resistant antimicrobial organisms as staphylococci (1-9) and tuberculosis. (10) Problem staph infection is persistent, insidious threat to hospitalized patients. Tuberculosis remains one of the technical and clinical problems of drug-resistant forms are becoming more common worldwide. (10.11) can use many difficult to treat, life-threatening microorganisms as weapons of terrorism is a necessary need for safe antimicrobial agents. As an example of the latter, spores of anthrax bacilli have recently been used as a large number of victims of weapons. (12-15)

To state the obvious, the best therapeutic antimicrobial drugs are virtually no side effects, have a wide range of activities, and probably will not encounter resistance to their therapeutic effect over time. The number of natural products, including several essential oils and some fats (monoglycerides) seems to have some of these ideal characteristics. Essential oils are used for food preservation since the early explorers. (16) Thus, it is not very surprising that they were found to be effective for many pathogenic organisms. (16-22) Some of monoglycerides and fatty acids, also found that the antimicrobial potential. (23) lauric acid is the main antiviral and antibacterial substance in human breast milk. Coconut products and palm oil are the main sources of this medium chain saturated fatty acids. Monolaurin, glycerin, ether laurynovoy acids, more biologically active than laurynovoy acid. Optimal antimicrobial activity for fatty acids and their corresponding monoglycerides are chain length C12. (24). Lauric acid (C12) has a higher antiviral activity than caprylic acid (C8), kaprinovaya acid (C10) or myristic acid (C14). (24)

We conducted a series of in vitro and in vivo studies to determine the potential of essential oils and fat monolaurin alone or in combination for treatment of various organisms. (25-27) with 12 essential oils tested, oregano consistently fairer treatment for the organism studied. (26.27) We also confirmed that the fat monolaurin is cidal in a variety of organisms. (26.27)

We used a micro-broth dilution technique to determine the sensitivity of Candida Albicans and various bacterial strains of oregano oil and other essential oils and monolaurin. (25-27) susceptibility was expressed as the minimum inhibitory and / or minimal cidal concentration. The minimum inhibitory concentration was defined as the lowest concentration of agent required to arrest the growth of fungi or bacteria, after 24 hours incubation. Minimum cidal concentration determined by seeding 0. 01 ml aliquots of culture medium taken from the tube after 48 hours on agar and incubated for further growth. Plates were scored for growth of colonies of bacteria. The lowest concentration of antibacterial agent causing negative growth (fewer than three colonies) was considered the minimum cidal concentration. Using the above methodology, we examined in vitro cidal and / or static effects of oregano oil, a few other essential oils and monolaurin for Candida Albicans, Staphylococcus aureus, bacillus anthrax Stern (model B. anthrax), Escherichia coli, Klebsiella pneumonia, helicobacter, and Mycobacterium Terrae (model for Mycobacterium tuberculosis). As oil oregano consistently proved the most effective essential oil, we will limit our discussion of the essential oil. Oil of oregano has been effective enough in reasonable concentrations relative to C. Albicans. (25) was found to completely inhibit the growth of C. Albicans in culture for 250 ug / ml. In contrast, monolaurin in our hands was not effective against the fungus. In the study of bacteria, oregano oil has proved cidal to all tested organisms mentioned above, except for B. anthrax Stern, for which it was static. (27) monolaurin was cidal to Staphylococcus aureus and M. Terrae but not E. coli and K. pneumonia. It was noted that well-monolaurin is mainly against gram-positive bacteria rather than Gram-negative. However, H.pylori were extremely sensitive to monolaurin contrast to two other gram-negative microorganisms. As oil of oregano, monolaurin was static, not cidal B. Stern anthrax. ? "Prepared to" How can we put these results in perspective, because many of us were inclined vitro treatments that never Consequently, the first question we must answer the following: whether it is a natural substance effectively in vivo? In first approximation, we investigated the therapeutic effectiveness of oregano oil in the experimental murine model of systemic candidiasis. (25) Groups of six mice infected with C. Albicans (5 X [LD. Sat. 50]) and fed different amount of oil oregano in a finite volume 0. 1 ml of olive oil (vehicle). Daily administration 8. 6 mg in 100 ml of oregano olive oil / kg body weight for 30 days resulted in 80% survival without renal burden of C. Albicans in comparison with a group of mice that received only olive oil. The latter died within 10 days. Next, 14 mice were infected with Staphylococcus aureus and injected daily with oil, oregano, monolaurin, or combined oregano and monolaurin within 30 days. (26) of bacterial agents administered was calculated based on body weight of mice. Control mice received daily gavages or an olive oil (negative control) or olive oil orally plus vancomycin (400 mg), F (positive control). Daily dose of oregano was 3 years. 2 mg. Monolaurin was given in the same dose, and when both drugs were combined, the same individual doses were used, i. e, 3. 2 mg each. Experiments were terminated at the end of 30 days. We found that oregano oil and monolaurin is protective, at least to some extent in vivo. (26) In 14 rats used as controls. Staphylococcus aureus (ATSS # 14775) killed all order strattera mice within 7 days. On the other hand, 37. 5% -50% of mice survived for 30 days after receiving daily gavages vancomycin (7/14), oregano oil (6/14) and monolaurin (4/8). Survival when combined oregano oil and monolaurin was a little better - 62. 5% (5/8). It is clear that further research to establish whether the combination of superior natural products for personal use, especially if higher doses of individual agents are used. Interestingly, no abscesses were found in postmortem and Staphylococcus aureus can be grown with kidney 30-day survivors offering treatment. .